V beta specificity of superantigen TSST-1 plus CD28 costimulation without APCs

Little has so far been known about the role of antigen-presenting cells (APCs) in the activation of T cells by superantigens. Recently several studies showed that superantigens could directly activate purified T cells in the presence of CD28 costimulation. Here we investigate V beta expression of T cells activated by the superantigen toxic shock syndrome toxin-1 (TSST-1), plus CD28 costimulation, in the absence of APCs. The aim of this study was to ask if TSST-1 activated purified T cells in the presence of CD28 costimulation with the same specificity as in the presence of APCs. We provide evidence that the specificity of TSST-1 to human V beta, in the presence of CD28 costimulation, is identical to that in the presence of APCs, with V beta 2 being significantly expanded. The results indicate that the main role of APCs in the superantigen-mediated T cell activation may be to provide T cells with CD28 costimulation.     

基于Android手机的人员定位与管理系统

提出来一种基于Android手机系统和Windows平台的人员定位与管理系统。该系统使用Android手机采集人员的位置和通信信息,通过手机GPRS网络将信息传送给服务器。管理者通过浏览程序访问服务器,获取人员的位置信息及与特定人员进行通信。系统是使用手机移动设备对人员进行管理的初步尝试,并且能建立高效的人员管理程序。     

数字化校园数据备份体系建设探索

随着高校信息化建设的推进,特别是数字化校园的广泛应用,重要数据越来越多地集中存储于学校数据中心。如何保障数据的安全成为亟待解决的问题。数据备份是确保数据安全的重要措施,本文分析了数据备份面临的挑战及应对方法,介绍了数据本地、异地备份的常用方法,设计了一套实用的异地数据灾备体系,并对未来的技术发展进行了展望。     

人白细胞介素-24基因的克隆、表达和纯化

目的对人白细胞介素-24(hIL-24)进行克隆、表达和纯化。方法分离人外周血单个核细胞,ConA刺激培养,提取细胞总RNA,应用RT-PCR技术从外周血单个核细胞中扩增hIL-24成熟肽编码区,定向克隆至融合表达载体pGEX-4T-1,重组载体pGEX-4T-1/hIL-24经DNA测序鉴定后,转化E.coliBL21(DE3),IPTG诱导表达,阴离子交换层析纯化融合蛋白,SDS-PAGE和Westernblot鉴定。结果所得hIL-24基因经序列分析,与GenBank公布一致。所构建融合表达载体pGEX-4T-1/hIL-24测序正确,转化BL21(DE3)后,37℃诱导表达相对分子质量约为44000的融合蛋白,主要以包涵体形式存在,占菌体总蛋白的30.63%。经纯化后,纯度达85%以上。Westernblot检测能与兔抗人IL-24的多克隆抗血清发生结合反应。结论已成功构建了hIL-24的融合表达载体pGEX-4T-1/hIL-24,并在大肠杆菌中高效表达,纯化后获得了高纯度的融合蛋白,为hIL-24的功能及活性研究奠定基础。     

磷钨酸/SiC复合材料的制备及光催化性能研究

以磷钨酸(PTA)和Si C为原料,采用浸渍法制备了4种不同比例PTA的PTA-Si C光催化剂。采用紫外可见漫反射光谱、傅里叶红外变换光谱、X射线衍射光谱、扫描电镜进行表征,并以罗丹明B(Rh B)为降解底物,考察光催化剂的光催化降解性能。结果表明,制备的光催化剂既保持了Si C的载体结构,又保持了PTA的Keggin型结构。PTA的质量分数40%的PTA-Si C光催化降解性能最好,在光照180 min时,Rh B的降解率为66%,光降解反应速率常数为5.21×10^-3min^-1。羟基自由基在光催化降解Rh B中起决定作用的活性物质。     

校园网内IP地址盗用技术及防范措施

IP地址盗用行为是目前校园网管理所面对的一大难题。该文以IP地址盗用的概念及常见盗用方法为出发点,着重介绍了如何针对不同的盗用方式实施有效的防范措施,并对各种防盗措施进行了适用性分析。     

计算机图形学的基本算法实现研究

该文对计算机图形学中多边形剪裁和逐点生成的基础算法进行比较和研究,对于提高计算机图形系统的性能具有很大的帮助,具有很强的理论和现实意义。     

Detection of urinary biomarkers for early diagnosis of acute renal allograft rejection by proteomic analysis

Acute allograft rejection has been recognized as a major impediment to improved success in renal transplantation. Timely detection and control of rejection are very important for the improvement in long-term renal allograft survival. Thus, biomarkers for early diagnosis of acute rejection are required urgently to clinical medication. This study seeks to search for such biomarker candidates by comparing patients' pre-treatment urinary protein profiling with their post-treatment urinary protein profiling. A total of 15 significantly and consistently down-regulated protein candidates were identified. Among them, alpha-1-antichymotrypsin precursor (AACT), tumor rejection antigen gp96 (GP96) and Zn-Alpha-2-Glycoprotein (ZAG) were selected for further analysis. The results indicated that Western Blot assay of AACT, GP96 and ZAG had advanced the diagnosis time of acute renal rejection by 3 days, compared with current standard clinical observation and laboratory examination. Furthermore, the double-blind detection revealed that the accuracy, sensitivity and specificity of the diagnosis of acute renal rejection of AACT, GP96 and ZAG were 66.67%/100%/60%, 83.33%/100%/80% and 66.67%/100%/60%, respectively, and 100%/100%/100% in combination. In conclusion, urinary protein AACT, GP96 and ZAG could be a set of potential biomarkers for early non-invasive diagnosis of the acute rejection after renal transplantation.     

基于支持向量机的思维脑电信号特征分类研究

探索一种实用的基于想象运动思维脑电的脑-机接口(BCI)方式,为实现BCI应用奠定比较坚实的理论和实验基础。对6名受试者进行三种不同时段(箭头出现2s、1s和0s后提示按键)情况下想象左右手运动思维作业的信号采集实验,利用小波变换和支持向量机对实验数据进行离线处理。对三种情况下的延缓时间△t0、△t1和△t2分析发现:△t0与△t1和△t2之间都有显著性差别(p<0.05),而△t1与△t2之间没有显著差别(p>0.05);平均分类正确率分别达到68.00%、80.00%和56.67%(p<0.05);实际按键前0.5~1s左右,想象左右手运动的思维脑电特征信号都发生了明显改变。通过合理的实验设计获取的信号有助于识别正确率的提高,为BCI系统中思维任务的特征提取与识别分类提供了新思路和方法。